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of genome integrity. They can be important for DNA replication and repair service in all organisms. Ligases were important reagents in the development of molecular cloning and plenty of subsequent ramifications of DNA biotechnology, together with molecular diagnostics and SOLiD sequencing strategies. Ligases are classy and functional enzymes and are having fun with a exploration renaissance in light of discoveries that almost all organisms have various ligases that either functionality in DNA replication (by joining Okazaki fragments) or are committed to certain DNA repair service pathways, for example nucleotide excision restore, base excision fix, single-strand crack restore, or even the maintenance of double-strand breaks by way of nonhomologous conclusion joining (two–four).
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). The NTase area binds for the damaged and intact DNA strands in the most important groove flanking the nick in addition to inside the minimal groove within the three′-OH facet in the nick (supplemental Fig. S3). The OB area binds throughout the slight groove to the experience on the duplex driving the nick.
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